Spore Collection Process
“Plant roots appear unchanged even though they are chock full of one or more fungi and so they must be cleaned and stained to see them clearly and to measure how much they have grown and spread.
Professor of Environmental Microbiology
Gigaspora margarita (top left)
Gigaspora gigantea (top right)
Network of hyphae and spores (bottom left)
Rhizophagus intraradices (bottom right)
“A germinating spore of
Gigaspora margarita, which is easy to see because of of the spore’s large size.
The spore sends out multiple ‛germ tubes’ (or hyphae), which are attracted to a root
surface. Each hypha penetrates directly into the root and begins a symbiosis with
the plant host. Applying individual spores to a seedling root and allowing the plant
to grow for 3–4 months is the best way to obtain a pure culture of the fungus.
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